ArticleRomagnani S, Maggi E, Biagiotti R, Giudizi GM, Amadori A, Ricci M.
Clin Exp Immunol. 1978 May;32(2):324-32.
Receptors for IgM were detected on peripheral blood and tonsil human lymphocytes by a rosette technique with ox red blood cells (ORBC) coated with anti-ORBC rabbit IgM. It was found that the receptors are very sensitive to handling procedures of cells and to low temperatures. An overnight incubation period at 37 degrees C was the optimal condition for the maximum expression of receptors for IgM, but the use of IgM-free media in these cultures was neither an essential nor favourable factor for an optimal rosette formation, when ORBC heavily coated with rabbit IgM were used. The great majority of chronic lymphocytic leukaemia (CLL) patients presented a high number of EA(IgM)-RFC, either on freshly drawn or cultured lymphocytes. By fractionation procedures of normal peripheral blood and tonsil lymphocytes, it was found that a subpopulation of B cells, like T cells, also posses a lower avidity for IgM than those present on T lymphocytes.